We recently collaborated on this piece of work from members of Max Cryle’s group (Monash/EMBL Australia). Using a range of techniques including protein X-ray crystallography, isothermal titration calorimetry and activity assays, this work characterises the structure and function of the C-terminal domain of the nonribosomal peptide synthetase Ebony. Results show that this C-terminal region encodes a eukaryotic example of an alternative type of nonribosomal peptide synethetase condensation domain, and experiments identified how this protein is able to provide selectivity for both the carrier protein-bound amino acid and the amine substrates.
You can find the paper here: https://www.pnas.org/content/116/8/2913.abstract
Well done to everyone that worked on this paper!
JACKSON RESEARCH GROUP 